Monoclonal antibodies against human necrosis factor (tnf) _...

C - Chemistry – Metallurgy – 12 – P

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167/139, 167/37,

C12P 21/08 (2006.01) A61K 39/395 (2006.01) C07K 16/24 (2006.01) C12N 5/18 (2006.01) G01N 33/566 (2006.01) G01N 33/577 (2006.01)

Patent

CA 1337717

The invention relates to hybridoma cell lines which synthesize highly specific monoclonal antibodies (mAb) against human tumor necrosis factor (H-TNF), to monoclonal antibodies against H-TNF having an affinity constant for H-TNF greater than 109 l/mol, which does not cross react with lymphotoxin and neutralizing the cytotoxic activities of H-TNF, to methods isolating and detecting H-TNF in samples using said antibodies, and to pharmaceutical compositions which contain them. The present invention also relates to the hybrid cell line ECACC 87 050801 and to the monoclonal antibody AM-195 produced by this hybrid cell line. The present invention also relates to methods for isolating and detecting human tumor necrosis factor-alpha using the AM-195 antibody. The present invention further relates to pharmaceutical compositions which contain the monoclonal antibody AM-195.

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