C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/08 (2006.01) C07K 16/30 (2006.01) C12N 5/18 (2006.01) G01N 33/574 (2006.01) G01N 33/577 (2006.01)
Patent
CA 2128285
ABSTRACT The present invention provides novel hybridoma cell lines which produce monoclonal antibodies that bind epi- topes found in the free form of Prostate Specific Antigen (PSA) but not in PSA complexed to .alpha.1-antichymotrypsin. PSA is a serine protease with a molecular mass of 33 kDa found as a specific cancer marker of prostatic cancer. The antibodies recognize their epitopes on the zymogen as well as on the active enzyme but the epitope is hidden by their specific inhibitor .alpha.1-antichymotrypsin (ACT), nor- mally found in human serum. The hybridomas are produced by fusing an immortal cell, a cell having the ability to replicate indefinitely in myeloma cell culture (NS-1, P3x63-Ag8,45.6 Tg), and an effector immune cell following immunization of the immune host with PSA isolated from patient material. The monoclonal antibodies produced by the hybridoma cell lines of the present invention are use- ful in the detection of free form of PSA which is impor- tant in screening of the patient material to distinguish carcinoma of the prostate (CAP) from less fatal benign prostatic hyperplasia (BHP).
Lilja Hans
Matikainen Marja-Terttu
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
Wallac Oy
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