Monoclonal antibodies against ricin toxin and methods of...

A - Human Necessities – 61 – K

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A61K 39/395 (2006.01) A61M 5/20 (2006.01) C07K 16/16 (2006.01)

Patent

CA 2533379

Disclosed herein are monoclonal antibodies (Mabs) against ricin toxin and its subunits. The Mabs were initially selected based upon their ability to bind to ricin and its individual subunits in a solid-phase enzyme-linked immunoassay (ELISA). Several candidates were selected for further evaluation, including their ability to inhibit ricin intoxication in vitro and for their utility as immunodiagnostic reagents. As disclosed herein, the Mabs may be used in immunoassays. Also disclosed, are use of the Mabs to inhibit ricin-mediated eukaryotic cell cytotoxicity in vitro, thereby indicating that these Mabs may be used to prevent and treat ricin intoxication in vivo. Kits comprising the Mabs are also disclosed.

L'invention concerne des anticorps monoclonaux (Mabs) dirigés contre la toxine de ricin et ses sous-unités. Ces Mabs ont été initialement sélectionnés sur la base de leur capacité à se lier au ricin et à ses sous-unités individuelles dans un dosage immunoenzymatique (ELISA) en phase solide. Plusieurs candidats ont été sélectionnés pour être évalués au sujet de leur capacité à inhiberin vitro une intoxication au ricin et pour leur utilité en tant que réactifs immunodiagnostiques. Les Mabs selon l'invention peuvent être utilisés dans des dosages immunoenzymatiques. L'invention concerne également l'utilisation de ces Mabs pour inhiber in vitro la cytotoxicité des cellules eucaryotes médiées par le ricin de façon à indiquer que ces Mabs peuvent être utilisés en vue de prévenir et de traiter in vivo une intoxication au ricin. L'invention concerne également des trousses comprenant ces Mabs.

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