Monoclonal antibodies capable of recognizing gangliosides...

Details Monoclonal antibodies capable of recognizing gangliosides... Monoclonal antibodies capable of recognizing gangliosides...

C - Chemistry, Metallurgy

12

P

167/129, 195/1.1

C12P 21/08 (2006.01) A61K 39/39 (2006.01) C07K 16/18 (2006.01) C12N 5/20 (2006.01)

Patent

CA 1335654

A monoclonal antibody specifically recognizes an epitope or an antigenic determinant represented by the following formula and specifically reacts therewith: Image The monoclonal antibody can be obtained by cultivating hybridoma cells which can be generated by a method comprising cell-fusing (A) B cells or lymphocytes obtained by immunizing an animal with a substance involving the epitope or the antigenic determinant represented by the foregoing formula and (B) myeloma cells. The monoclonal antibody shows high specificity to ganglioside GQ1b and ganglioside GT1a having a specific epitope and thus can detect the epitope in high sensitivity. Therefore, the antibody can be used for the elucidation of sugar chain's roles in cell functions and for studying the development of animals. Moreover, the monoclonal antibody would have various clinical applications.

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