Monoclonal antibodies directed against lg4/5 domain of...

C - Chemistry – Metallurgy – 07 – K

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C07K 16/18 (2006.01) A61K 39/395 (2006.01) A61P 35/00 (2006.01) C12N 5/16 (2006.01) C12N 15/13 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2747064

The present invention relates to a monoclonal antibody binding to the LG4/5 domain of chain alpha3 of human protein laminin-5, wherein said monoclonal antibody inhibits the binding of syndecan-1 to said laminin-5 alpha3 chain LG4/5 domain, in particular 1H12 monoclonal antibody produced by the hybridoma cell line named 1H12 deposited on January 8, 2008 at the C. N. C. M. under number 1-3890, as well as chimerized, humanized derivatives and fragments thereof, and nucleic acid sequences encoding them, as well as vectors and host cells expressing them. The invention further relates to the medical application of such antibodies, in particular for treating cancer.

La présente invention concerne un anticorps monoclonal se liant au domaine LG4/5 de la chaîne alpha3 de la protéine humaine laminine-5, ledit anticorps monoclonal inhibant la liaison du syndécane-1 audit domaine LG4/5 de la chaîne alpha3 de la laminine-5, en particulier l'anticorps monoclonal 1H12 produit par la lignée cellulaire de l'hybridome appelée 1H12 déposée le 8 janvier 2008 au C. N. C. M. sous la référence 1-3890, ainsi que ses fragments et dérivés chimérisés et humanisés, et les séquences d'acide nucléique les codant, de même que les vecteurs et cellules hôtes les exprimant. La présente invention concerne en outre l'application médicale de tels anticorps, en particulier dans le traitement du cancer.

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