C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
167/139, 167/37,
C12N 5/18 (2006.01) A61K 39/395 (2006.01) C07K 16/30 (2006.01) G01N 33/574 (2006.01) G01N 33/577 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2007506
ABSTRACT OF THE INVENTION MONOCLONAL ANTIBODIES DIRECTED TO TUMOR-ASSOCIATED GANGLIOSIDES AND FUCOGANGLIOSIDES AND METHOD FOR PRODUCTION THEREOF A method of producing monoclonal antibodies that bind to tumor-associated gangliosides, the method comprising: (1) immunizing a host with tumor cells; (2) boosting said host with a suspension comprising a mixture of tumor cell membrane and at least one purified lactonized tumor-associated ganglioside; (3) boosting said host with an immunogen comprising a lactone of a tumor associated ganglioside adsorbed on or incorporated into a carrier; (4) fusing immunized cells from said host with myeloma cells to form hybridoma cells; (5) selecting hybridoma cells that produce antibody that binds to said ganglioside of step (3); (6) culturing said selected hybridoma cells; and (7) recovering said antibody. Hybridomas and monoclonal antibodies produced by the above-described method. A passive immunization method for treatment of tumors containing gangliosides comprising administering to a subject: (A) a pharmaceutically effective amount of an antibody produced by the above described method. A method for detecting tumors containing gangliosides comprising: (A) contacting a test sample with an antibody produced by the above described method; and (B) assaying for the specific binding of said antibody to antigen in said test sample. - 45 -
Clausen Henrik
Hakamori Sen-Itiroh
Nudelman Edward
Singhal Anil
Biomembrane Institute (the)
G. Ronald Bell & Associates
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