Monoclonal antibodies reactive with defined regions of the t...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/00 (2006.01) A61K 35/18 (2006.01) A61K 39/395 (2006.01) C07K 16/28 (2006.01) C07K 16/46 (2006.01) G01N 33/564 (2006.01) G01N 33/566 (2006.01) G01N 33/577 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2143186

The invention is directed to monoclonal antibodies reactive with a member of the V.beta.3 family variable region of the beta chain of the TCR. More particularly, the invention provides for detection of the V.beta.3.1 subfamily. In a specific embodiment the invention provides for detection of V.beta.3.1, without cross-reacting with other V.beta.3 family variable regions. In a specific embodi- ment, the monoclonal antibodies of the invention do not react with V.beta.3.2. In particular, the invention provides monoclonal antib- odies, termed 5E4 and 8F10, which react with the variable region of a member of the V.beta.3 family. In various embodiments of the invention, these antibodies, or fragments or derivatives thereof, can be used to bind with a member of the V.beta.3 family TCR var- iable region amino acid sequences, either as part of an intact TCR or peptide, or T cell-surface molecule, or a fragment thereof. The monoclonal antibodies are useful for diagnosis and therapy of autoimmune disease, in particular rheumatoid arthritis.

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