Monoclonal antibodies specific for the extracellular domain...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) A61K 39/395 (2006.01) A61K 47/48 (2006.01) A61K 51/00 (2006.01) C07K 14/00 (2006.01) C07K 14/705 (2006.01) C07K 16/00 (2006.01) C07K 16/18 (2006.01) C07K 16/28 (2006.01) C07K 19/00 (2006.01) C12N 5/12 (2006.01) G01N 33/554 (2006.01) G01N 33/60 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2323096

The present invention relates to monoclonal antibodies that bind to the extracellular domain of prostate-specific membrane antigen (PSMA), hybridoma cell lines producing the antibodies, and methods of using such antibodies for diagnosis and treatment of cancer. In particular, thirty-five monoclonal antibodies reactive with PSMA expressed on the cell surface are exemplified. Additionally, the present invention relates to a novel protein variant (PSM') of PSMA detected by a number of the antibodies of the invention. The hydrolase activity of PSMA and PSM' allows the use of an immunoenzymatic assay for their detection.

La présente invention concerne des anticorps monoclonaux se liant au domaine extracellulaire de l'antigène d'enveloppe prostatique spécifique(PSMA), des lignées cellulaires hybridomes produisant les anticorps et des méthodes d'utilisation de ces anticorps pour le diagnostic et le traitement du cancer. En particulier, 35 anticorps monoclonaux réactifs avec un PSMA exprimé à la surface cellulaire servent d'exemples. L'invention concerne en outre un nouveau variant de protéine (PSM') de PSMA détecté par plusieurs des anticorps de l'invention. L'activité hydrolase de PSMA et PSM' permettent d'utiliser un dosage immunoenzymatique pour les détecter.

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