Monoclonal antibodies to egg yolk immunoglobulins (igy)

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/16 (2006.01) C07K 16/42 (2006.01) G01N 33/566 (2006.01) G01N 33/573 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2167214

Chicken antibodies have grown in popularity for use in immunoassays due to several advantages over mammalian antibodies. Using purified IgG from chicken yoke a series of murine monoclonal antibodies were developed and characterized by ELISA and immunoblotting techniques. The monoclonal antibodies obtained have a high affinity to chicken IgG, specifically the heavy chain of IgG. No cross reactivity with human, murine, goat or rabbit IgG was observed. These monoclonal antibodies have been used as reporter antibodies and have also been conjugated to horse radish peroxidase without any apparent loss in specificity or affinity for chicken IgG.

Les anticorps du poulet sont utilisés de plus en plus fréquemment dans des immuno-essais à cause de différents avantages par rapport aux anticorps de mammifères. On a produit une série d'anticorps monoclonaux murins en utilisant des IgG purifées du jaune d'oeuf de poulet; ces anticorps ont été caractérisés par la méthode ELISA et par la méthode de transfert électrophorétique suivi d'une immunodétection. Les anticorps monoclonaux obtenus ont une forte affinité pour l'igG de poulet, plus particulièrement pour la chaîne lourde de l'IgG. Il n'y a pas de réaction croisée avec des igG humaines, murines, de chèvre ou de lapin. Ces anticorps monoclonaux ont été utilisés comme anticorps rapporteurs et ils ont également été conjugués à la peroxydase du raifort sans perte apparente de spécificité ou d'affinité pour l'IgG de poulet.

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