Monoclonal antibodies to gastrin hormone

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 16/26 (2006.01) A61K 39/395 (2006.01) C12N 5/20 (2006.01) G01N 33/577 (2006.01) G01N 33/74 (2006.01)

Patent

CA 2561405

The present invention provides monoclonal antibodies (MAbs) selective for the N-termini and C-termini of the gastrin hormone forms, gastrin-17 (G17), glycine-extended gastrin-17 (G17-Gly), gastrin-34 (G34) and glycine-extended gastrin-34 (G34-Gly); and the hybridomas that produce these MAbs. Also provided are panels of MAbs useful for the detection and quantitation of gastrin-17 (G17), glycine-extended gastrin-17 (G17-Gly), gastrin-34 (G34) and glycine-extended gastrin-34 (G34-Gly). These assays are useful for monitoring a gastrin-mediated disease or condition, or for monitoring the progress of a course of therapy. The invention further provides solid phase assays including immunohistochemical (IHC) and immunofluorescence (IF) assays suitable for detection and visualization of gastrin species in solid samples, such as biopsy samples or tissue slices. Pharmaceutical compositions of the MAbs of the invention are also provided, along with methods of diagnosis, prevention and treatment of gastrin-mediated diseases or conditions. Methods of evaluating a gastrin hormone-blocking treatment are described. The course of a gastrin-mediated disease or condition may be monitored in a patient by means of assay methods provided.

La présente invention concerne des anticorps monoclonaux (MAb) sélectifs des teminaux N et des terminaux C de diverses formes de l'hormone gastrine, soit gastrine-17 (G17), gastrine-17 à extension glycine (G17-Gly), gastrine-34 (G34) et gastrine-34 à extension glycine (G34-Gly), ainsi que hybridomes produisant ces MAb. L'invention concerne également des panneaux de MAb utiles pour la détection et la quantification de la gastrine-17 (G17), de la gastrine-17 à extension glycine (G17-Gly), de la gastrine-34 (G34) et de la gastrine-34 à extension glycine. Ces analyses conviennent pour la surveillance de pathologies ou d'états induits par la gastrine, ou pour la surveillance de l'évolution d'une thérapie en cours. De plus, l'invention porte sur des essais en phase solide comprenant des essais immunohistochimiques (IHC) et des essais par immunofluorescence (IF) convenant pour la détection et la visualisation d'espèces gastrine, tels que des échantillons pour biopsie et des coupes biologiques. L'invention concerne également des compositions pharmaceutiques renfermant les Mab de l'invention ainsi que des méthodes de diagnostic, de prévention et de traitement des pathologies ou des états induits par la gastrine. Sont décrites des méthodes d'évaluation d'un traitement de blocage de l'hormone gastrine. Les méthodes d'essais décrites conviennent pour la surveillance de l'évolution de pathologies ou d'états induits par la gastrine chez un patient.

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