Monoclonal antibodies useful in the identification of...

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167/37, 195/1.10

G01N 33/577 (2006.01) G01N 33/569 (2006.01)

Patent

CA 1282691

MONOCLONAL ANTIBODIES USEFUL IN THE IDENTIFICATION OF MICROORGANISMS CAUSING PERIODONTAL DISEASE ABSTRACT Bacteroides gingivalis frequently has been implicated in adult periodontitis. The present monoclonal antibodies are specific to Bacteroides gingivalis. The present monoclonal antibodies are typically employed as a reagent which includes an inert carrier, preferably a liquid such as buffered solution and a preservative. The carrier compositions are suitably selected to provide for the proper dispersal of bacteria, and to preserve the integrity of antigens and supplemental structures. The selection of the proper carrier is especially important in the detection of bacteria which produce large amounts of autolytic enzymes such as B. gingivalis. The monoclonal antibodies of the present invention are useful in clinical testing and differentiating antigens in the gingival or subgingival sera. The method of testing and differentiating involves the steps of contacting a sample of the bacterial flora from a lesion or other site with a measured amount of a monoclonal antibody specific to a single antigen site on Bacteroides gingivalis and subsequently measuring the number of antibody-antigen complexes formed.

528540

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