Monoclonal antibody 1a7 and use for the treatment of...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/13 (2006.01) A61K 31/70 (2006.01) A61K 39/395 (2006.01) A61K 48/00 (2006.01) C07H 21/04 (2006.01) C07K 14/52 (2006.01) C07K 14/535 (2006.01) C07K 14/55 (2006.01) C07K 16/42 (2006.01) C07K 16/46 (2006.01) C07K 19/00 (2006.01) C12N 5/18 (2006.01) C12Q 1/68 (2006.01) G01N 33/564 (2006.01) G01N 33/574 (2006.01) G01N 33/577 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2210158

The present invention relates to monoclonal antibody 1A7. This is an anti-idiotype produced by immunizing with an antibody specific for ganglioside GD2, and identifying a hybridoma secreting antibody with immunogenic potential in a multi-step screening process. Also disclosed are polynucleotide and polypeptide derivatives based on 1A7, including single chain variable region molecules and fusion proteins, and various pharmaceutical compositions. When administered to an individual, the 1A7 antibody overcomes immune tolerance and induces an immune response against GD2, which comprises a combination of anti-GD2 antibody and GD2-specific T cells. The invention further provides methods for treating a disease associated with altered GD2 expression, particularly melanoma, neuroblastoma, glioma, soft tissue sarcoma, and small cell carcinoma. Patients who are in remission as a result of traditional modes of cancer therapy may be treated with a composition of this invention in hopes of reducing the risk of recurrence.

L'invention porte sur l'anticorps monoclonal 1A7. Il s'agit d'un anti-idiotype produit par immunisation par un anticorps spécifique du ganglioside GD2 et identification d'un anticorps sécrétant un hybridome présentant un potentiel immunogène dans un procédé de dépistage à plusieurs étages. Sont également présentés des dérivés de polynucléotides et de polypeptides du 1A7 comportant des molécules monocaténées de région variable et des protéines de fusion et différentes préparations pharmaceutiques. Lorsqu'on l'administre à un individu, l'anticorps 1A7 surmonte la tolérance immunitaire et provoque une réponse immunitaire vis à vis du GD2 qui comprend un mélange d'anticorps anti-GD2 et de cellules T spécifiques du GD2. L'invention porte en outre sur des méthodes de traitement de maladies associées à une expression modifiée du GD2 et en particulier du mélanome, du neuroblastome, du gliome, du sarcome des tissus mous, ou du carcinome des petites cellules. Les patients en rémission suite à une thérapie usuelle anticancéreuse traités par la susdite composition peuvent espérer une réduction du risque de rechute.

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