Monoclonal antibody against apolipoprotein a-1

Details Monoclonal antibody against apolipoprotein a-1 Monoclonal antibody against apolipoprotein a-1

C - Chemistry, Metallurgy

12

N

C12N 15/08 (2006.01) C07K 16/18 (2006.01) C12N 5/12 (2006.01) C12P 21/08 (2006.01) G01N 33/53 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2356738

A monoclonal antibody reacting specifically with (1) apoA-I having a molecular weight of not more than 150,000 and occurring in HDL free from apoA-II; and (2) apoA-I not binding to a lipid; a hybridoma producing this antibody; a method of immunologically assaying apoA-I characterized by reacting the antibody with a specimen; and an assay reagent for apoA-I which contains the antibody. The specific apoA-I thus assayed is usable as a novel indication of lipid metabolic error, etc.

L'invention concerne un anticorps monoclonal qui réagit surtout en présence (1) d'une apoA-I ayant un poids moléculaire ne dépassant pas 150.000 et ayant lieu dans HDL en l'absence de apoA-II ; et (2) d'une apoA-I n'étant pas liée à un lipide. L'invention concerne aussi un hybridome produisant cet anticorps, un procédé d'analyse immunologique d'apoA-I consistant à faire réagir l'anticorps avec un échantillon; et un réactif d'analyse pour l'apoA-I contenant l'anticorps. L'apoA-I ainsi analysée sert à indiquer les erreurs métaboliques lipides, entre autres.

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