Monoclonal antibody and gene encoding the same, hybridoma,...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/13 (2006.01) A61K 9/127 (2006.01) A61K 39/395 (2006.01) A61K 47/48 (2006.01) A61P 35/00 (2006.01) C07K 16/30 (2006.01) C07K 16/32 (2006.01) C12N 5/00 (2006.01) C12N 5/16 (2006.01) C12N 15/00 (2006.01) C12N 15/63 (2006.01) C12P 21/08 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2515389

A novel monoclonal antibody specifically recognizing cancer cells of, for example, non-small cell lung cancer, pancreatic cancer or gastric cancer is produced by hybridomas which are obtained by fusing lymphocytes originating in a cancer tissue of a cancer patient with mouse myeloma cells. A remedy for cancer is obtained by using the antibody alone or in the state of being carried on the surface of a liposome having a toxin or an anticancer drug encapsulated therein. More specifically speaking, a remedy for cancer is obtained by using an antibody, wherein the heavy chain variable region contains the amino acid sequence of SEQ ID NO:115 and the light chain variable region contains the amino acid sequence of SEQ ID NO:117, either alone or in the state of being carried on the surface of a liposome having a toxin or an anticancer drug encapsulated therein.

La présente invention a trait à un anticorps monoclonal d'identification spécifique de cellules cancéreuses, par exemple, de cancer bronchopulmonaire <=non à petites cellules>=, de cancer du pancréas produit par des hybridomes obtenus par la fusion de lymphocytes issus d'un tissu cancéreux d'un patient souffrant de cancer avec des cellules de myélome de souris. Un remède contre le cancer est obtenu par l'utilisation de l'anticorps seul ou transporté à la surface d'un liposome comportant une toxine ou un médicament contre le cancer encapsulé à l'intérieur. De manière plus spécifique, un remède contre le cancer est obtenu par l'utilisation d'un anticorps, dans lequel la région variable de chaîne lourde contient la séquence d'acides aminés de SEQ ID NO :115 et la région variable de chaîne légère contient la séquence d'acides aminés de SEQ ID NO :117, seul ou transporté à la surface d'un liposome comportant une toxine ou un médicament contre le cancer encapsulé à l'intérieur.

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