Monoclonal antibody and immunoassay using the same

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 16/42 (2006.01) C12N 5/10 (2006.01) C12P 21/08 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2734704

Disclosed are: an anti-human immunoglobulin M monoclonal antibody which can react with human immunoglobulin M specifically to form an immunological aggregate through the antigen-antibody reaction with the human immunoglobulin M in a solution; an immunoassay using the monoclonal antibody; an inhibitor which can inhibit a non-specific reaction induced by human immunoglobulin M which cannot be inhibited by the conventional techniques; and an immunoassay in which a non-specific reaction induced by human immunoglobulin M is inhibited. In the selection of a monoclonal antibody capable of reacting with human immunoglobulin M by employing the reactivity in a solution as a measure for the evaluation, it becomes possible to produce a novel monoclonal antibody which can cause the aggregation of human immunoglobulin M by itself and enables a practical immunoagglutination measurement.

La présente invention porte sur : un anticorps monoclonal anti-immunoglobuline M humaine qui peut réagir avec une immunoglobuline M humaine de façon spécifique pour former un agrégat immunologique par la réaction antigène-anticorps avec l'immunoglobuline M humaine dans une solution; un immunodosage faisant intervenir l'anticorps monoclonal; un inhibiteur qui peut inhiber une réaction non spécifique induite par une immunoglobuline M humaine qui ne peut pas être inhibée par les techniques classiques; et un immunodosage dans lequel une réaction non spécifique induite par une immunoglobuline M humaine est inhibée. Lors de la sélection d'un anticorps monoclonal capable de réagir avec une immunoglobuline M humaine grâce à la réactivité dans une solution en tant que mesure pour l'évaluation, il devient possible de produire un nouvel anticorps monoclonal qui peut provoquer l'agrégation d'immunoglobuline M humaine en soi et permettre une mesure d'immunoagglutination pratique.

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