Monoclonal antibody-containing agent

A - Human Necessities – 61 – K

Patent

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Details

A61K 47/42 (2006.01) A61B 10/00 (2006.01) A61K 39/395 (2006.01) C12N 5/20 (2006.01) C12N 15/06 (2006.01) C12P 21/08 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2054302

Abstract of the Disclosure The agent of the present invention comprising a monoclonal antibody which is specifically linked to a protein encoded by human chromosome 21 and which is linked to a protein whose expression in brain varies dependent upon age or a protein which is expressed in only specific cells of brain can be obtained as follows. Mice are immunized with CHO cells containing long arms of human chromosome 21 (2Fur cells), then the resulting antibody-producing cells are fused with mouse myeloma cells and hybridomas which produce monoclonal antibodies capable of specifically recognizing a protein encoded by human chromosome 21 are selected. A hybridoma which produces a monoclonal antibody capable of recognizing a protein expressed in the human brain tissues is selected from the resulting hybridomas and then the hybridoma is proliferated to give the intended monoclonal antibody.

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