Monoclonal antibody detection of proteins on sperm related...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/18 (2006.01) C07K 1/22 (2006.01) C07K 14/47 (2006.01) C07K 14/705 (2006.01) C07K 16/18 (2006.01) C07K 16/28 (2006.01) G01N 33/577 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2223381

Heparin binding proteins (HBP) are produced by male accessory glands and bind to sperm at ejaculation. The ability of accessory glands to produce HBP and sperm to bind HBP differs among males. Identifying the presence of a 21.5, 24 and 30 kDa heparin binding proteins in sperm membranes with a monoclonal antibody resulted in separating groups of bulls by 40 % difference in fertility. Thus, fertility potential of a bull can be predicted by heparin binding protein content of sperm membranes.

Des protéines liant l'héparine (HBP) sont produites par les glandes annexes mâles et se lient au sperme lors de l'éjaculation. La faculté des glandes annexes de produire des HBP et du sperme de se lier au HBP diffère selon les mâles. L'identification de la présence de protéines de liaison d'héparine de 21,5, 24 et 30 kDa dans des membranes de spermatozoïdes à l'aide d'un anticorps monoclonal a permis de séparer des groupes de boeufs présentant une différence de fécondité de 40 %. Ainsi, il est possible de prédire le potentiel de fécondité d'un boeuf selon la teneur en protéines de liaison d'héparine de membranes de spermatozoïdes.

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