Monoclonal antibody, method of production thereof and use...

Details Monoclonal antibody, method of production thereof and use... Monoclonal antibody, method of production thereof and use...

C - Chemistry, Metallurgy

12

P

C12P 21/08 (2006.01) C07K 16/44 (2006.01) C12N 5/18 (2006.01) G01N 33/577 (2006.01) G01N 33/88 (2006.01)

Patent

CA 2072412

24205-935 ABSTRACT OF THE DISCLOSURE Disclosed are a monoclonal antibody which specifically recognizes 6,15-diketo-13,14-dihydro-prostaglandin F1.alpha. with high sensitivity, a hybridoma producing the monoclonal antibody, a method of preparing the hybridoma and an immunoassay method for 6,15-diketo-13,14-dihydro-prostaglandin F1.alpha. using the monoclonal antibody. The monoclonal antibody makes it possible to conveniently measure with specificity and high sensitivity 6,15- diketo-13,14-dihydro-prostaglandin F1.alpha. that is a stable metabolite of prostaglandin I2. The monoclonal antibody is useful in determining the content of prostaglandin I2, which exhibits platelet aggregating inhibitory activity, vasodilating activity and other activities, in biological samples.

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