Monoclonal antibody recognizing fk506-binding protein method...

Details Monoclonal antibody recognizing fk506-binding protein method... Monoclonal antibody recognizing fk506-binding protein method...

C - Chemistry, Metallurgy

07

K

C07K 16/28 (2006.01) G01N 33/566 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2142197

The present invention relates to a monoclonal antibody (anti-FKBP antibody) which recognizes an antigenic determinant in an FK506-binding protein and does not inhibit the binding between an FK506-binding protein and FK506, a method for assaying an FKBP level in plasma, comprising reacting an immobilized anti-FKBP antibody, an enzyme-labelled FK506 and FKBP in a specimen, and assaying the degree of color development of an enzyme substrate, and a kit for the assay. According to the present invention, the FKBP level in plasma which affects the immunosuppressive action of FK506 can be assayed more precisely with ease, which in turn enables setting the optimal dose of FK506 in close accordance with the condition of individual patients.

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