Monoclonal antibody specific to neutrophils

Details Monoclonal antibody specific to neutrophils Monoclonal antibody specific to neutrophils

C - Chemistry, Metallurgy

12

P

195/1.11

C12P 21/08 (2006.01) C07K 16/28 (2006.01) G01N 33/569 (2006.01)

Patent

CA 1283066

ABSTRACT A hybrid cell line capable of producing monoclonal antibodies uniquely specific to human neutrophils. The monoclonal antibody has no reactivity with other human peripheral blood cells and virtually no reactivity with granulocyte precursors or other cells in bone marrow. Further, there is no reactivity with human acute leukemia cells. One of the partners in the hybridoma fusion of a mouse spleen cell de- veloped from using highly purified human granulocytes as the im- munization agent. The monoclonal antibody further is character- ized by its capability of being used to enumerate and isolate neutrophils in normal peripheral blood and patients with acute leukemia.

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