C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/08 (2006.01) C07K 16/18 (2006.01) C07K 16/44 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2056407
2056407 9015329 PCTABS00002 A competitive method of measuring human ventricular myosin light chain in cardiac patients which comprises incubating at least a monoclonal or polyclonal anti-HVLCS antibody, an enzyme, a VLCS antigen and an unknown amount of HVLCS analyte present in patient's serum. The anti-HVLCS antibody or the VLCS antigen, is directly or indirectly detected by means of enzyme-bound label, whereby the amount of HVLCS analyte, when initially present in patient's serum, is determined by comparing the extent to which the said VLCS antigen is bound to the said anti-HVLCS antibody with a calibration curve obtained from a known amount of said antigen. The anti-HVLCS antibody or the VLCS antigen is solid-phase bound. The anti-HVLCS monoclonal antibody has specificity to at least one of HVLC1 and HVLC2. There is prepared a monoclonal antibody which specifically binds to VLC1 and which is produced by the hybridoma cell line having the ATCC accession number HB 10471.
Hebert Manon
Kowalik Maria
Sikorska Hanna
Rougier Inc.
Swabey Ogilvy Renault
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