Monoclonal antibody specifically reactive with human perforin

Details Monoclonal antibody specifically reactive with human perforin Monoclonal antibody specifically reactive with human perforin

C - Chemistry, Metallurgy

12

P

C12P 21/08 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) C12N 5/20 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2049883

ABSTRACT The present invention provides a hybridoma capable of producing a monoclonal antibody reacting specifically with human perforin and the monoclonal antibody belonging to subclass IgG2b and having an L chain of the K type. The hybridoma can be produced by (i) integrating a base sequence encoding an amino acid sequence from position 297 (inclusive) to position 463 (inclusive) of human perforin into an expression vector pET3C at the BamHI restriction site, the expression vector carrying the following 35 base sequence which partially constitutes the BamHI restriction site: Image (ii) transforming E. coli cells with the thus modified expression vector, (iii) allowing the resultant transformant to produce a fused partial human perforin, (iv) immunosensitizing a rodent with the fused partial human perforin, and (v) fusing a spleen cell or lymphonodus cell obtained from the sensitized rodent with a mouse myeloma cell to prepare the hybridoma.

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