C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
167/129, 167/37
C12P 21/00 (2006.01) C07K 14/37 (2006.01) C07K 16/14 (2006.01) C12N 15/00 (2006.01) G01N 33/53 (2006.01)
Patent
CA 1266233
MONOCLONAL ANTIBODY TO CANDIDA ALBICANS CYTOPLASMIC ANTIGENS AND METHODS OF PREPARING SAME Abstract Two novel hybridoma cell lines, ATCC #HB-8397 and ATCC #HB-8398 produce monoclonal antibody monospecific to a single determinant shared by a set of three closely related cytoplasmic antigens of Candida albicans. The antigens have molecular weights of 120-135 Kd, 48-52 Kd, and 35-38 Kd. The hybridomas are formed by fusing splenocytes from immunized BALB/c mice with SP2/O-Ag 14 myeloma cells. Monoclonal and monospecific, polyclonal antibodies to these cytoplasmic antigens find application in the immunodiag- nosis of Candida infections. A procedure is provided for preparing partially purified cytoplasmic antigen of pathoganic Candida species for administration to splenocyte-donating mice. Also provided is a method for the biochemical purification of cytoplasmic antigen of a pathogenic Candida species used for the preparation of monoclonal and monospecific, polyclonal antisera thereto.
587720
Buckley Helen R.
Largen Michael T.
Strockbine Nancy A.
Buckley Helen R.
Gowling Lafleur Henderson Llp
Largen Michael T.
Strockbine Nancy A.
Temple University Of The Commonwealth System Of Higher Education
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