Monoclonal antibody to enterohemorrhagic escherichia coli...

C - Chemistry – Metallurgy – 12 – P

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C12P 21/08 (2006.01) C07K 14/245 (2006.01) C07K 16/12 (2006.01) C12N 5/18 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2066777

A monoclonal antibody specific for enterohemorrhagic Escherichia coli 0157:H7 and 026:H11 is produced by immunizing BALB/c mice with a strain of E. coli 0157:H7: The antibody reacts strongly by an enzyme-linked inmmunosorbent assay with an approximately 5,000 - 6;000 dalton molecular weight outer membrane protein of strains of enterohemorrhagic Escherichia coli 0157:H7 and 026H11: A rapid and sensitive assay for detecting these organisms is also disclosed.

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