Monoclonal antibody to human glicentin, hybridoma for...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/18 (2006.01) C07K 16/26 (2006.01) G01N 33/543 (2006.01) G01N 33/577 (2006.01) G01N 33/74 (2006.01)

Patent

CA 2161480

A monoclonal antibody to human glicentin having a recognition site in the C-terminal region of the positions from 51 to 69, especially from 51 to 62 in the amino acid sequence of human glicentin. The antibody is produced by a hybridoma 3D5A (FERM BP-5214) which is produced by cell fusion of antibody-producing cells immunized with human glicentin and myeloma cells. The antibody is of high sensitivity to human glicentin and advatageously used for a determination or assay for human glicentin by a sandwich method in combination with an antibody to human glicentin having a recognition site different from that of the above mentioned antibody, preferably in the N-terminal region.

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