Monoclonal antibody which recognizes a specific...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/18 (2006.01) C07K 16/30 (2006.01)

Patent

CA 2049938

A hybridoma cell line is provided which is capable of producing a monoclonal antibody which binds to a unique determinant site on the surface and in the cytoplasm of human breast carcinoma cells and carcinoma cells of other adenocarcinomas and does not bind selectively to the surface of normal human breast epithelial cells except in instances of high concentration of the antibody in the testing fluid. The cell line of the invention was developed by immunizing mice with a select group of immunogens and a conventional myeloma cell line for fusion with the murine splenocytes harvested. The monoclonal antibody is identified as the BrE3 monoclonal antibody. The BrE3 mononclonal antibody binds to an antigen which is characterized as high molecular weight mucin-like glycoprotein complex having a molecular weight exceeding 400,000 daltons that is bound by a disulfide bond to a protein of 69,000 daltons. The BrE3 mononclonal antibody is especially useful for diagnostic detection, prognostic, and possible therapeutic applications in human breast cell carcinoma.

L'invention concerne une lignée cellulaire d'hybridomes capable de produire un anticorps monoclonal se liant à un site déterminant unique sur la surface et dans le cytoplasme de cellules du cancer du sein humain et de cellules du carcinome d'autres adénocarcinomes, et ne se liant pas sélectivement à la surface de cellules épithéliales du sein humain normales, sauf dans des cas de concentration élevée de l'anticorps dans le fluide de test. On a développé la lignée cellulaire de l'invention par immunisation d'une souris à l'aide d'un groupe sélectionné d'immunogènes, et d'une lignée cellulaire de myélomes classiques, permettant une fusion avec les splénocytes murins recueillis. L'anticorps monoclonal est identifié comme étant l'anticorps monoclonal BrE3. Ledit anticorps monoclonal BrE3 se lie à un antigène, lequel est caractérisé comme étant un complexe de masse moléculaire élevée de glycoprotéines analogues à la mucine, dont la masse moléculaire dépasse 400 000 daltons, lequel est lié par une liaison de bisulfure à une protéine de 69 000 daltons. Ledit anticorps monoclonal BrE3 est particulièrement utile dans le diagnostic, le pronostic, et dans des applications thérapeutiques possibles dans le carcinome cellulaire du sein humain.

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