C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/62 (2006.01) C07K 1/107 (2006.01) C07K 14/435 (2006.01) C12N 15/12 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 21/64 (2006.01)
Patent
CA 2481328
The present invention relates generally to fluorescent proteins and fluorescent protein variants, and more specifically to monomeric and dimeric forms of Anthozoan fluorescent proteins. In one aspect, the present invention provides variants of fluorescent proteins, where the variants have a reduced propensity to tetramerize, and form dimeric or monomeric structures. In a further aspect, the present invention provides variants of fluorescent proteins, the variants being characterized by more efficient maturation than corresponding fluorescent proteins from which they are derived. The invention also relates to methods of making and using such fluorescent proteins and fluorescent protein variants, including fluorescent protein monomers and dimers.
L'invention concerne d'une manière générale des protéines fluorescentes et des variants protéiques fluorescents, plus précisément des formes monomères et dimères de protéines fluorescentes d'anthozoaires. Dans un aspect, l'invention concerne des variants de protéines fluorescentes, lesdits variants ayant une tendance à se tétramériser, et à former des structures dimères ou monomères. Dans un autre aspect, l'invention concerne des variants protéiques fluorescents, lesdits variants se caractérisant par une maturation plus efficace que les protéines fluorescentes correspondantes d'où ils découlent. L'invention concerne également des procédés de fabrication de tels protéines fluorescentes et variants protéiques fluorescents, y compris des monomères et dimères protéiques fluorescents.
Baird Geoffrey S.
Campbell Robert E.
Tsien Roger Y.
Mbm Intellectual Property Law Llp
The Regents Of The University Of California
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