C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/00 (2006.01) A01H 4/00 (2006.01) A01H 5/00 (2006.01) C12N 9/88 (2006.01) C12N 15/20 (2006.01) C12N 15/60 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2380221
cDNAs encoding gymnosperm monoterpene synthases have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding a monoterpene synthase of the invention. Thus, systems and methods are provided for the recombinant expression of recombinant monoterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts.
Selon cette invention, on a isolé et séquencé les ADNc codant pour les synthétases de monoterpène de Gymnospermes, et l'on a déterminé les séquences d'acides aminés correspondantes. L'invention se rapporte aussi à des cellules hôtes modifiées qui ont été transformées, transfectées, infectées et/ou qui ont reçu une injection avec un véhicule de clonage recombinant et/ou une séquence d'ADN codant pour une synthétase de monoterpène de l'invention. L'invention concerne enfin des systèmes et des procédés destinés à l'expression recombinante des synthétases de monoterpène recombinantes qui peuvent s'utiliser pour faciliter leur production, leur isolation et leur purification en quantités importantes.
Bohlmann Joerg
Croteu Rodney B.
Phillips Michael A.
Steele Christopher L.
Mbm Intellectual Property Law Llp
Washington State University Research Foundation
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