Mouse monoclonal antibodies to hiv-1p24 and their use in...

C - Chemistry – Metallurgy – 12 – P

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167/37, 195/1.10

C12P 21/08 (2006.01) C07K 16/10 (2006.01) G01N 33/577 (2006.01)

Patent

CA 1340919

The present invention provides monoclonal antibodies demonstrating specific reactivity with HIV-I p24. One monoclonal antibody designated 31-42-19 recognizes an unique epitope on HIV-I p24 that is not immunogenic in humans. 31-42-19 also reacts with an antigenically cross reactive epitope on HIV-2 p24. Another monoclonal antibody designated 31- 90-25 recognizes an epitope within a highly immunogenic region of HIV-I p24. The present invention also provides cell lines capable of producing these monoclonal antibodies. The invention also includes a highly sensitive enzyme immunoassay for the detection of HIV-I p24 in biological fluids, using a monoclonal antibody mixture. The present invention further provides methods for the use of these monoclonal antibodies for the detection of anti-HIV-I p24 antibodies and HIV-2 p24 antigen in biological samples.

602167

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