Mrna transfected antigen presenting cells

Details Mrna transfected antigen presenting cells Mrna transfected antigen presenting cells

C - Chemistry, Metallurgy

07

H

C07H 21/04 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2546378

A method of amplifying RNA to obtain RNA molecules that are predominantly in the sense orientation and essentially devoid of RNA molecules that are in the anti-sense orientation. A method of transfecting antigen presenting cells with a composition comprising sense RNA encoding immunogenic antigens and essentially devoid of antisense RNA and dsRNA is also provided as well as dendritic cells prepared according to the method.

Procédé d'amplification d'ARN pour obtenir des molécules d'ARN ayant principalement une orientation sens et essentiellement exemptes d'orientation anti-sens. Un procédé de transfection de cellules présentatrices d'antigène avec une composition contenant de l'ARN sens codant des antigènes immunogènes et essentiellement exempte d'ARN anti-sens et d'ARNds est également décrit, ainsi que des cellules dendritiques préparées selon ledit procédé.

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