Multi-color laser scanning confocal imaging system

G - Physics – 02 – B

Patent

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G02B 21/00 (2006.01)

Patent

CA 2067188

A true multi-color laser scanning confocal imaging system for use with a microscope is described in which a single laser having a multi-line output is used to simultaneously or individually excite a plurality of dyes. The images may be simultaneously viewed by a plurality of photomultiplier tubes to reconstruct an image showing distinct features of a specimen stained with different dyes. The images may also be constructed using a single detector is a time-multiplex fashion and using a computer to construct the image.

L'invention se rapporte à un système d'imagerie multicouleur de type réel à foyer commun et à balayage laser, qui est conçu pour être utilisé avec un microscope et dans lequel on utilise un seul laser comportant un faisceau de sortie multiligne pour exciter simultanément ou individuellement plusieurs colorants. Les images peuvent être visualisées simultanément par plusieurs tubes photomultiplicateurs, de façon à reconstruire une image présentant distinctement les caractéristiques de l'échantillon coloré par différents colorants. La construction des images peut également s'effectuer grâce à l'utilisation d'un seul détecteur en mode multiplex dans le temps et grâce à l'utilisation d'un ordinateur qui construit l'image.

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