Multi-plasmid system for the production of influenza virus

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 7/01 (2006.01) A61K 39/145 (2006.01) C07K 14/11 (2006.01) C12N 7/00 (2006.01) C12N 7/04 (2006.01) C12N 15/09 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2626266

Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics.

L'invention porte: sur des vecteurs et des méthodes de production de virus de la grippe utilisables comme vaccins de recombinaison contre la grippe dans des cultures de cellules; sur des vecteurs d'expression bidirectionnelle utilisables dans un système d'expression de virus de la grippe à plasmides multiples; sur des méthodes de production de virus de la grippe à capacité accrue de réplication dans des oeufs de poule embryonnés et/ou des cellules (par exemple Vero et/ou MDCK); et sur des virus de la grippe à capacité accrue de réplication.

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