Multi-purpose blood diluent and lysing agent for...

Details Multi-purpose blood diluent and lysing agent for... Multi-purpose blood diluent and lysing agent for...

G - Physics

01

N

150/13, 150/5

G01N 33/96 (2006.01) G01N 15/12 (2006.01) G01N 33/50 (2006.01) G01N 33/72 (2006.01)

Patent

CA 1158967

ABSTRACT A multipurpose isotonic blood diluent and a method for use of this diluent with a weak lysing reagent system, which is especially suitable for enumeration of traditional hemogram values, and also the determination of lymphoid-myeloid populations of leukocytes, particularly in automatic particle counting systems to yield two-volume leukocyte histograms. This blood diluent is an osmotically balanced aqueous solution of preselected pH containing Procaine hydrochloride for maintaining erythrocyte morphology during operation, N-(2-acetamido)iminodiacetic acid (ADA) as a blood cell stabilizing agent, and bacteriostatic agents including sodium 1-hydroxyphridine-2-thione, dimethylolurea and chlorhexidene diacetate which, together with the ADA, allow preferential determination of myeloid-lymphoid leukocytes, and other hematological values. The lysing agent is a mixture of an aqueous solution of at least one quaternary ammonium salt, having surface active properties, and an alkali metal cyanide, being present in a concentration range which is effective itself as a lysing agent and to give two volume leukocyte histograms.

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