Multianalyte molecular analysis

C - Chemistry – Metallurgy – 40 – B

Patent

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Details

C40B 30/04 (2006.01) C12Q 1/68 (2006.01) C40B 20/04 (2006.01) C12P 19/34 (2006.01) C40B 50/14 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2413978

The present invention provides methods and apparatus for the application of a particle array in bioassay format to perform qualitative and/or quantitative molecular interaction analysis between two classes of molecules (an analyte and a binding agent). The methods and apparatus disclosed herein permit the determination of the presence or absence of association, the strength of association, and/or the rate of association and dissociation governing the binding interactions between the binding agents and the analyte molecules. The present invention is especially useful for performing multiplexed (parallel) assays for qualitative and/or quantitative analysis of binding interactions of a number of analyte molecules in a sample.

La présente invention concerne des méthodes ainsi qu'un appareil destinés à l'application d'un réseau de particules en un format de biodosage pour exécuter une analyse d'interaction moléculaire qualitative et/ou quantitative entre deux classes de molécules (un analyte et un agent de liaison). Les méthodes et l'appareil de l'invention permettent la détermination de la présence ou de l'absence d'association, la force de l'association, et/ou le taux d'association et de dissociation gouvernant les interactions de liaison entre les agents de liaison et les molécules d'analyte. La présente invention est particulièrement utile pour exécuter des dosages multiplexés (parallèles) pour l'analyse qualitative et/ou quantitative d'interactions de liaison d'un certain nombre de molécules d'analyte dans un échantillon.

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