Multicopy-integration of heterologous genes and expression...

Details Multicopy-integration of heterologous genes and expression... Multicopy-integration of heterologous genes and expression...

C - Chemistry, Metallurgy

12

N

C12N 15/74 (2006.01) A01N 63/00 (2006.01) C12P 21/02 (2006.01) C12Q 1/00 (2006.01)

Patent

CA 2553485

The integration of genes into methylobacterium, such as M. extorquens ATCC 55366 is disclosed, using a transposon system, preferably the mini-Tn7 transposon system, and under the control of a promoter, such as the strong methanol dehydrogenase promoter (P mxaF). Multicopy integration of genes of interest is also disclosed. The unique and specific attachment site for the Tn7 attachment (attTn7) is identified for M. extorquens.

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