C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/11 (2006.01) A01H 5/00 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2583334
The present invention relates to methods and constructs for delivering double stranded RNA (dsRNA) to pest organisms. More specific, the invention relates to a multidomain RNA molecule consisting of a nucleotide sequence comprising (i) at least one aptamer and (ii) at least one nucleotide sequence of interest forming double-stranded RNA, said double-stranded RNA comprising annealed complementary strands, one of which comprises a nucleotide sequence which is complementary to at least part a pest target nucleotide sequence. The invention further relates to nucleic acids encoding the multidomain RNA molecules and to various uses of the multidomain RNA molecules in agriculture.
L'invention concerne des procédés et des produits de synthèse permettant d'administrer un ARN en double brin (RNAdb) à des organismes de parasites. Plus précisément, l'invention concerne une molécule d'ARN multidomaine comprenant une séquence nucléotidique renfermant : (i) au moins un aptamère et (ii) au moins une séquence nucléotidique étudiée formant un ARN en brin double, celui-ci comprenant des brins complémentaires recuits, un de ceux-ci présentant une séquence nucléotidique complémentaire d'au moins partiellement une séquence nucléotidique cible de parasites. L'invention concerne également des acides nucléiques codant les molécules d'ARN multidomaine et diverses utilisations des molécules d'ARN multidomaine en agriculture.
Bogaert Thierry Andre Olivier Eddy
Feldmann Pascale
Plaetinck Geert
Devgen N.v.
Smart & Biggar
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