Multiflavor streptavidin

C - Chemistry – Metallurgy – 07 – H

Patent

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Details

C07H 21/04 (2006.01) C07K 14/00 (2006.01) C07K 14/36 (2006.01) C12N 15/00 (2006.01)

Patent

CA 2283716

Compounds and methods are described for producing streptavidin mutants with changed affinities. In particular, modifications to the sequence of the natural streptavidin gene is described to create amino acid substitutions resulting in greater affinity for biotin subsitutes than for biotin.

L'invention concerne des composés et méthodes de production de mutants de streptavidine présentant des affinités modifiées. L'invention concerne en particulier, des modifications de la séquence du gène de streptavidine naturel pour créer des substitutions d'acides aminés présentant une meilleure affinité pour des substituts de biotine que pour la biotine elle-même.

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