Multifunctional enzyme

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/64 (2006.01) A61K 35/12 (2006.01) A61K 38/48 (2006.01) C12N 9/00 (2006.01) C12N 9/48 (2006.01) C12N 9/66 (2006.01) G02C 13/00 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2212533

The invention relates to a multifunctional enzyme that can be derived from crustaceans or fish. The enzyme has at least one of a chymotrypsin, trypsin, elastase, collagenase and exo peptidase activity, and a molecular weight between about 20 kd and about 40 kd as determined by SDS PAGE. Preferably, the multifunctional enzyme has substantial anti cell-cell adhesion activity. Preferably, the multifunctional enzyme has substantial homology with the krill multifunctional enzyme. These enzymes are useful for treating viral infections such as herpes outbreaks, fungal, bacterial or parasitic infections, including the primary and secondary infections of leprosy, colitis, ulcers, hemorrhoids, corneal scarring, dental plaque, acne, cystic fibrosis, blood clots, wounds, immune disorders including autoimmune disease and cancer. Additionally, the invention relates to a method of purifying the multifunctional enzyme, and to a preparation of essentially purified multifunctional enzyme.

L'invention concerne une enzyme à fonctions multiples pouvant être obtenue à partir de crustacés ou de poissons. Cette enzyme possède au moins une parmi les activités de chymotrypsine, trypsine, élastase, collagénase et exo peptidase et un poids moléculaire situé entre 20 kd et 40 kd, comme déterminé SDS PAGE: De préférence, cette enzyme présente une homologie sensible avec l'enzyme à fonctions multiples du krill. Ces enzymes sont utiles pour traiter des infections virales, telles que les éruptions d'herpès, des infections mycosiques, bactériennes ou parasitaires, y compris les infections primaires et secondaires de la lèpre, la colite, les ulcères, les hémorroïdes, la tache cornéenne, la plaque dentaire, l'acné, la mucoviscidose, les caillots sanguins, les blessures, les maladies immunes, y compris les maladies auto-immunes et le cancer. De plus, l'invention concerne un procédé de purification de l'enzyme à fonctions multiples, ainsi qu'une préparation constituée de l'enzyme purifiée.

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