Multifunctional recombinant phycobiliprotein-based...

C - Chemistry – Metallurgy – 07 – K

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C07K 19/00 (2006.01) C07K 1/14 (2006.01) C07K 14/415 (2006.01)

Patent

CA 2392712

The invention provides multifunctional fusion constructs which are rapidly incorporated into a macromolecular structure such as a phycobilisome such that the fusion proteins are separated from one another and unable to self- associate. The invention provides methods and compositions for displaying a functional polypeptide domain on an oligomeric phycobiliprotein, including fusion proteins comprising a functional displayed domain and a functional phycobiliprotein domain incorporated in a functional oligomeric phycobiliprotein. The fusion proteins provide novel specific labeling reagents.

L'invention concerne des gènes chimères de fusion multifonctionnels qui s'incorporent rapidement dans une structure macromoléculaire telle qu'un phycobilisome, de façon à séparer les protéines de fusion et à les empêcher de s'auto-associer. L'invention concerne des procédés et des compositions permettant de présenter un domaine de polypeptide fonctionnel sur une phycobiliprotéine oligomère, y compris des protéines de fusion comportant un domaine fonctionnel présenté et un domaine de phycobiliprotéine fonctionnel incorporés dans une phycobiliprotéine oligomère fonctionnelle. Les protéines de fusion constituent de nouveaux réactifs de marquage spécifique.

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