G - Physics – 01 – N
Patent
G - Physics
01
N
150/11, 167/41
G01N 33/78 (2006.01) G01N 33/538 (2006.01) G01N 30/52 (2006.01)
Patent
CA 1140465
ABSTRACT OF THE DISCLOSURE A specific binding assay method, and a test device and test kit for use therein, for determining a ligand, such as an antigen or antibody, in, or the ligand binding capacity of, a liquid medium, particularly a body fluid such as serum, wherein the unknown ligand competes with a labeled component, such as a radiolabeled form of the ligand or of a binding analog of the ligand, for binding with a binding partner, and wherein separation of the resulting bound-species and free-species of the labeled component is accomplished by allowing the liquid reaction mixture to be drawn by capillary action into a column comprising a bed of an adsorbent material selective for one of the two species. The improvement comprises using a column con- taining at least one additional bed of capillarily absorbent material disposed above said adsorbent bed and which is sub- stantially nonabsorbent relative to the one of said two species which said adsorbent bed selectively binds. The use of speci- fic types of capillarily absorbent beds in addition to the selectively adsorbent bed to form multilayer columns overcomes several unforeseen problems encountered in using the pre- viously known column chromatography method for certain assay applications.
353304
Feingers Judith
Pick Anthony J.
Wagner Daniel B.
Ames-Yissum Ltd.
Osler Hoskin & Harcourt Llp
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