Multimer sample pretreatment and immunoassay without...

G - Physics – 01 – N

Patent

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G01N 33/53 (2006.01)

Patent

CA 2542825

It is intended to provide a method of pretreating a sample for conveniently, quickly and accurately assaying the total amount of adiponectin, which occurs as a mixture of various multimer species in a biological sample, by an immunological assay method. Namely, a method of pretreating a sample for assaying adiponectin in order to immunologically assay the total amount of adiponectin in a sample, characterized by comprising treating the sample containing adiponectin with at least one member selected from among a reducing agent, an acid or its salt, a surfactant and a protease.

L'invention porte sur un procédé de traitement d'un échantillon de façon à doser de manière appropriée, rapidement et avec précision la quantité totale d'adiponectine qui se présente sous forme d'un mélange de diverses espèces multimères dans un échantillon biologique, selon une méthode de dosage immunologique. L'invention porte notamment sur un procédé de prétraitement d'un échantillon pour doser l'adiponectine afin d'effectuer un dosage immunologique de la quantité totale de l'adiponectine dans un échantillon, ce procédé étant caractérisé en qu'il consiste à traiter l'échantillon contenant l'adiponectine avec au moins un élément sélectionné parmi un agent de réduction, un acide ou son sel, un tensioactif et une protéase.

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