C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/62 (2006.01) C07K 14/195 (2006.01) C07K 14/435 (2006.01) C07K 19/00 (2006.01) C12N 15/11 (2006.01) C12N 15/12 (2006.01) C12N 15/31 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2627545
Multimeric tryptophan biosensors are disclosed, which comprise tryptophan- binding domains conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon tryptophan binding. Such biosensors are useful for real time monitoring of tryptophan metabolism in living cells.
La présente invention concerne des biocapteurs multimétriques au tryptophane, qui comprennent des domaines de liaison au tryptophane conjugués à des fractions donneuses et fluorescentes qui permettent la détection et la mesure du Transfert d~Energie de Résonance par Fluorescence lors d~une liaison au tryptophane. De tels biocapteurs sont utiles pour une surveillance en temps réel du métabolisme du tryptophane dans les cellules vivantes.
Frommer Wolf B.
Kaper Thijs
Carnegie Institution Of Washington
Gowan Intellectual Property
LandOfFree
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