Multiparametric fluorescence in situ hybridization

C - Chemistry – Metallurgy – 07 – H

Patent

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Details

C07H 21/04 (2006.01) C12N 15/12 (2006.01) C12N 15/31 (2006.01) C12N 15/33 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2329253

The invention relates to a set of combinatorially labeled oligonucleotide probes each member thereof: (i) having a predetermined label distinguishable from the label of any other member of said set, and (ii) being capable of specifically hybridizing with a predetermined chromosome or nucleic acid molecule, and to the use of such molecules, alone or in concert with nucleic acid amplification methods.

Cette invention a trait à un ensemble de sondes oligonucléotidiques marquées de manière combinatoire dont chaque élément, (i), est porteur d'une marque prédéterminée pouvant être distinguée de celle de tout autre élément dudit ensemble et, (ii), est capable d'hybridation spécifique avec un chromosome ou une molécule d'acide nucléique prédéterminés. Elle porte également sur l'utilisation de ces molécules, seules ou associées à des techniques d'amplification d'acide nucléique.

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