Multiparametric fluorescence in situ hybridization

Details Multiparametric fluorescence in situ hybridization Multiparametric fluorescence in situ hybridization

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2241148

The invention relates to a set of combinatorially labeled oligonucleotide probes each member threof: (i) having a predetermined label distinguishable from the label of any other member of said set, and (ii) being capable of specifically hybridizing with one predetermined autosomal or sex chromosome of a human karyotype, and to the use of such molecules to identify the individual autosomal and sex chromosomes of a human karyotype.

L'invention concerne un ensemble de sondes oligonucléotidiques marquées de manière combinatoire, chaque sonde (i) ayant un marqueur prédéterminé qui peut être distingué du marqueur d'un autre élément quelconque dudit ensemble et (ii) étant capable de former spécifiquement un hybride avec un chromosome autosomique prédéterminé ou un chromosome sexuel prédéterminé d'un caryotype humain. L'invention concerne également l'utilisation de ces molécules pour identifier les chromosomes autosomiques et sexuels prédéterminés d'un caryotype humain.

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