Multiple color staining reagent and method of analysis

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/02 (2006.01) G01N 33/53 (2006.01) G01N 33/533 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2065827

A multiple color staining reagent and method for blood cell analysis, especially through the use of flow cytometry, comprising two or more selected monoclonal antibodies coupled with a fluorescent label, particularly FITC and phycoerythrin, and an unlabeled, non-specific IgG antibody are provided. Specifically described is a multiple color staining reagent comprising a combination of two or more mouse monoclonal antibodies, each labeled with a different fluorescent dye, useful in double staining assays and the like for blood cell analysis.

Réactif de coloration multiple et méthode d'analyse des cellules sanguines faisant appel en particulier à la cytométrie de flux, comprenant deux ou plusieurs anticorps monoclonaux choisis couplés à un marqueur fluorescent, notamment FITC et la phycoérythrine, et un anticorps IgG non spécifique non marqué. Un réactif de coloration multiple est décrit, qui comprend une combinaison de deux ou plusieurs anticorps monoclonaux de souris marqués chacun au moyen d'une teinture fluorescente différente, utile dans les épreuves de double coloration et autres épreuves semblables pour l'analyse des cellules sanguines.

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