Multiple-container systems with improved sensitivity for...

B - Operations – Transporting – 01 – L

Patent

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Details

B01L 3/00 (2006.01) C12M 1/14 (2006.01) C12Q 1/68 (2006.01) G01N 21/05 (2006.01) G01N 33/48 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2351710

The invention relates to multiple-container systems and to the use of same for optical assays. The invention relates in particular to a multiple-container system comprising a container wall matrix (1) with continuous cavities and one or more optically transparent base plates (2) joined to the container wall matrix.aid system is characterized in that the base plates are coated with a film (3) having functional groups suitable for the covalent immobilization of molecules. The film on the base plate is preferably a Langmuir-Blodgett film, and preferably a Langmuir-Blodgett film on cellulose basis. The invention further relates to multiple-container systems comprising a container wall matrix with continuous cavities and one or more optically transparent base plates joined to the container wall matrix. Said systems are characterized in that the base plates are coated with a film derivatized with receptor molecules or molecules presenting low non-specific protein adsorption.

L'invention concerne des ensembles à plusieurs récipients, ainsi que l'utilisation de ces ensembles pour les analyses optiques. L'invention concerne en particulier un ensemble à plusieurs récipients comportant une matrice de paroi de récipient (1) pourvue de cavités traversantes et une ou plusieurs plaques de fond (2) optiquement transparentes montées sur ladite matrice. Cet ensemble à récipients multiples se caractérise en ce que la ou les plaques de fond sont recouvertes d'un film (3) présentant des groupes fonctionnels adaptés pour l'immobilisation par covalence de molécules. Le film placé sur la plaque de fond est, de préférence, un film Langmuir-Blodgett à base de cellulose. L'invention concerne en outre des ensembles à plusieurs récipients qui comprennent une matrice de paroi de récipient présentant des cavités traversantes et une ou plusieurs plaques de fond optiquement transparentes, montées sur ladite matrice. Ces ensembles se caractérisent en ce que la ou les plaques de fond sont recouvertes d'un film dérivatisé avec des molécules de récepteur ou d'autres molécules qui présentent une faible adsorption de protéine non spécifique.

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