Multiple marker characterization of single cells

Details Multiple marker characterization of single cells Multiple marker characterization of single cells

G - Physics

01

N

G01N 33/554 (2006.01) C12Q 1/68 (2006.01) G01N 33/569 (2006.01) G01N 33/574 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2350692

Methods use concurrent multiple cellular probes conjugated to fluorescent compounds of different wavelengths to characterize single cells that have been isolated from a body fluid using density gradient centrifugation. Specific antibodies, peptides, nucleotides or oligonucleotides are used as probes for both identification and characterization of a single cell.

L'invention concerne des méthodes mettant en oeuvre de multiples sondes cellulaires conjuguées à des composés fluorescents de différentes longueurs d'onde pour caractériser des cellules individuelles isolées d'un fluide corporel par centrifugation par gradient de densité. Des anticorps, peptides, nucléotides ou oligonucléotides spécifiques sont utilisés comme sondes tant pour l'identification que pour la caractérisation d'une cellule individuelle.

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