Multiple mode multiplex reaction quenching method

Details Multiple mode multiplex reaction quenching method Multiple mode multiplex reaction quenching method

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2579741

Methods for balancing multiplexed PCR reactions are provided which exploit differences in primer and amplicon Tms. The methods may be controlled by a computer process. Also provided are articles of manufacture useful in such methods and compositions containing primers and probes useful in such methods.

L'invention concerne des procédés permettant d'équilibrer des réactions PCR multiplexées, dans lesquels sont utilisées les différences entre les Tm des amorces et des amplicons. Ces procédés peuvent être contrôlés par un processus informatique. L'invention concerne également des articles fabriqués pouvant être utilisés dans ces procédés, ainsi que des compositions contenant des amorces et des sondes pouvant être utilisées dans ces procédés.

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