Multiple mutation variants of serine protease

Details Multiple mutation variants of serine protease Multiple mutation variants of serine protease

C - Chemistry, Metallurgy

12

N

C12N 9/52 (2006.01) C12N 15/52 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2667043

The present invention provides Micrococcineae spp serine proteases having multiple substitutions. In particular, the present invention provides serine proteases having multiple substitutions, DNA encoding these proteases, vectors comprising the DNA encoding the proteases, host cells transformed with the vector DNA, and enzymes produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising these serine protease variants. In particularly preferred embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These variant proteases also find use in numerous applications.

L'invention concerne des protéases de sérine de l'espèce Micrococcineae ayant des substitutions multiples. En particulier, la présente invention fournit des protéases de sérine ayant des substitutions multiples, de l'ADN codant ces protéases, des vecteurs comprenant l'ADN codant les protéases, des cellules hôtes transformées avec le vecteur ADN, et des enzymes produites par les cellules hôtes. La présente invention fournit également des compositions de nettoyage (par exemple des compositions de détergent), des compositions d'alimentation animale, des compositions de traitement de textile et de cuir comprenant ces variantes de protéase de sérine. Dans des modes de réalisation particulièrement préférés, la présente invention fournit des protéases mutantes (c'est-à-dire des variantes) provenant de protéases de type sauvage décrites ici. Ces protéases variantes trouvent également une utilisation dans de nombreuses applications.

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