Multiple reporter gene assay

C - Chemistry – Metallurgy – 12 – Q

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Details

C12Q 1/34 (2006.01) C12Q 1/66 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2241760

A method of measuring the activity of at least two reporter gene products in an aliquot of a sample extract is disclosed. The activities of a first and second reporter enzyme are quantified by measuring the light signal produced by degradation of a first substrate by the first reporter enzyme and the light signal produced by the degradation of a second substrate by a second reporter enzyme. Both quantifications are sequentially performed on the same aliquot of sample extract.

Méthode pour mesurer l'activité d'au moins deux produits de gènes marqueurs dans une aliquote d'un échantillon. On quantifie les activités d'un premier et d'un second enzyme marqueur en mesurant le signal lumineux produit par la dégradation d'un premier substrat par le premier enzyme marqueur et le signal lumineux produit par la dégradation d'un second substrat par un second enzyme marqueur. Les deux quantifications s'effectuent sur la même aliquote d'extrait d'échantillon._________________________________________

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